小鼠內脂素/內髒脂肪素(visfatin)ELISA試劑盒含税含运费 , Elisa试剂盒价格,Elisa试剂盒说明书,Elisa试剂盒技术,Elisa试剂盒售后,Elisa试剂盒免费代测详情咨询: 電 話: 手 機: 傳 真: 上海恒远生物科技有限公司长期提供各种种属、各种系列ELISA檢測試劑盒,价格实惠,质量有保证,信誉*。索取各ELISA试剂盒产品说明书。凡购买目录本公司ELISA檢測試劑盒可免费提供代测服务。本公司的更多产品,请点击公司:www.shhykit.com 産品規格:96T/48T 96T指可以做94個標本,2個標准對照 48T指可以做47個標本,1個標准對照 96T-84個樣本 48T-42個樣本 操作步驟 1.標准品的稀釋:本試劑盒提供原倍標准品一支,用戶可按照下列圖表在小試管中進行稀釋。 3600 pg/ml5号標准品150μl的原倍標准品加入150μl標准品稀释液 1800pg/ml4號標准品150μl的5號標准品加入150μl標准品稀釋液 900pg/ml3號標准品150μl的4號標准品加入150μl標准品稀釋液 450 pg/ml2号標准品150μl的3号標准品加入150μl標准品稀释液 225pg/ml1號標准品150μl的2號標准品加入150μl標准品稀釋液 2.加樣:分別設空白孔(空白對照孔不加樣品及酶標試劑,其余各步操作相同)、標准孔、待測樣品孔。在酶標包被板上標准品准確加樣50μl,待測樣品孔中先加樣品稀釋液40μl,然後再加待測樣品10μl(樣品zui終稀釋度爲5倍)。加樣將樣品加于酶標板孔底部,盡量不觸及孔壁,輕輕晃動混勻。 3.溫育:用封板膜封板後置37℃溫育30分鍾。 4.配液:將20倍濃縮洗滌液用蒸餾水20倍稀釋後備用 5.洗滌:小心揭掉封板膜,棄去液體,甩幹,每孔加滿洗滌液,靜置30秒後棄去,如此重複5次,拍幹。 6.加酶:每孔加入酶標試劑50μl,空白孔除外。 7.溫育:操作同3。 8.洗滌:操作同5。 9.顯色:每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鍾. 10.終止:每孔加終止液50μl,終止反應(此時藍色立轉黃色)。 11.测定:以空白空调零,450nm波长依序测量各孔的吸光度(OD值)。 测定应在加终止液后15分钟以内进行。 Assay procedure 1.Dilute and add sample:Dilute Original density Standard as follow table: 800 nmol/L5 Standard150μl Original density Standard+150μl Standard diluent 400 nmol/L4 Standard150μl 5 Standard+150μl Standard diluent 200 nmol/L3 Standard150μl 4 Standard+150μl Standard diluent 100 nmol/L2 Standard150μl 3 Standard +150μl Standard diluent 50 nmol/L1 Standard150μl 2 Standard +150μl Standard diluent 2.add sample:Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix. 3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃. 4.Configurate liquid: 30-fold wash solution diluted 30-fold (or 20-fold) with distilled water and reserve. 5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat. 6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except blank well. 7.incubate:Operation with 3. 8.washing:Operation with 5. 9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃ 10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color). 11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min. 小鼠內脂素/內髒脂肪素(visfatin)ELISA試劑盒免費代測,本試劑盒詳細說明書歡迎您來電索取。 |